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. 2003 Jun;2(3):510–520. doi: 10.1128/EC.2.3.510-520.2003

FIG. 1.

FIG. 1.

Characterization of scw1-18 and scw1Δ mutant strains. (A and B) scw1-18 (DM1559) cells from a log-phase culture at 30°C were fixed and stained with DAPI to visualize nuclei (A) or stained with Calcofluor to visualize cell wall and septa (B). (C) Wild-type (DM105), scw1-18 (DM1559), scw1Δ (DM1274), scw1-18/scw1+ (DM1300), and scw1-18/scw1Δ (DM1301) strains were grown in YE to mid-log phase at 30°C and then scored for the septation index. WT, wild type. (D) scw1-18 mutant cells (DM1559) containing either the pREP42 or pREP42-scw1+ plasmid were grown in EMM without uracil and thiamine for 24 h at 30°C, at which time the septation index was scored. (E) Alignment of the RNP domain of Scw1p with those of two related budding yeast proteins, Whi3 and Whi4. Conserved residues are marked with black boxes. The octamer RNP1 and hexamer RNP2 are labeled as previously defined (39).