FIG. 5.

The scw1-18 mutation can stabilize microtubules. (A) nda3-KM311 and scw1-18 nda3-KM311 cells were synchronized at early G₂ by centrifugal elutriation from log-phase cultures grown at 30°C. Synchronized cells were then shifted to 19°C, and septation was scored for both cultures at 1-h intervals. (B) DAPI-stained cells at the 9-h time point. scw1-18 nda3-KM311 mutant cells often only partially segregated their DNA, leading to anucleate cell compartments (arrows). DIC, differential interference contrast. (C) DAPI and tubulin staining with TAT1 antibody of asynchronous cells of the indicated genotypes 6 h after a shift to 19°C. (D) DAPI and tubulin staining with TAT1 antibody of cells treated with 25 mg of MBC per ml for 2 h at 30°C. WT, wild type. (E) Serial dilution patch test for growth of the indicated single and double mutant strains. Dilutions shown were 10-fold, starting with 10⁴ cells. Strains were pregrown in liquid YE at 25°C and then spotted onto YE plates or on YE with 10 mg of MBC per ml and incubated at the indicated temperatures for 3 to 5 days before photography.