FIG.4.

Chemical-induced cell cycle arrest and release in the presence of pheromone. (A) DNA content of cells arrested by chemical treatment. Wild-type FB1 cells were incubated in YPD (control), YPD with 1 mg of HU per ml, or YPD with 10 μM benomyl (Bn). Samples were removed after 90 min, and the DNA content was measured by FACS analysis. (B) Release of HU-arrested cells in the presence of pheromone. FB1c and TAU3 cells were grown in YPD to an OD₆₀₀ of 0.2 and then incubated for 90 min in the presence of 1 mg of HU per ml. After this incubation, cells were washed three times and then released in CM with synthetic a1 pheromone (0.1 ng/ml). Numbers indicate times after transfer to fresh medium without HU and pheromone. (C) Release of benomyl-arrested cells in the presence of pheromone. FB1c and TAU3 cells were grown in YPD to an OD₆₀₀ of 0.2 and then incubated for 60 min in the presence of 10 μM benomyl. After this incubation, cells were washed three times and then released in CM with synthetic a1 pheromone (0.1 ng/ml). Numbers indicate times after transfer to fresh medium without benomyl and pheromone.