TABLE 1.
Prestalk genes
| Accession no. | cDNA | Producta | Fold enrichment (Pst/Psp) |
|---|---|---|---|
| PstA genes | |||
| AU062194 | SLI271 | BCS1-like (3e-14) | 4.32 |
| AU061665 | SLF308 | Extracellular matrix protein (4e-05) | 3.02 |
| C94343 | SSK861 | Dictyostelium O-methyltransferase (4e-43) | 2.79 |
| PstAB genes | |||
| SLA128 pattern | |||
| AU060076 | SLA128 | Arabinofuranohydrolase (0.15) | 7 |
| AU060722 | SLB233 | Homologous to arabidopsis hypothetical protein (3e-09) | 5.99 |
| AU038850 | SSL558 | Dictyostelium cellulase (6e-09) | 4.11 |
| SLC388 pattern | |||
| AU060958 | SLC388 | Dictyostelium steroid isomerase | 4.29 |
| AU061846 | SLG322 | Carboxypeptidase (3e-16) | 3.26 |
| SSK348 pattern | |||
| AU062236 | SLI604 | Aconitate hydratase (4e-78) | 2.83 |
| AU074317 | SSK348 | Propionate-coenzyme A ligase (8e-30) | 2.22 |
| SSB312 pattern | |||
| AU060501 | SLK182 | ECMB homolog | 9.2 |
| AU071364 | SSB312 | Dictyostelium hypothetical protein AAL99319.1 (6e-11) (=SSC596) | 4.95 |
| C91223 | SSJ839 | Unknown | 4.35 |
| PstO genes | |||
| SSM184 pattern | |||
| AU038878 | SSL587 | Calcium binding protein 4A | 6.29 |
| AU072229 | SSD764 | Splicing factor 3A (0.007) | 3.58 |
| AU071425 | SSB476 | Unknown | 3.52 |
| AU037107 | SSB457 | Dictyostelium thioredoxin 1 | 3.26 |
| AU038948 | SSM184 | Unknown | 2.75 |
| AU073765 | SSI311 | Glycosylphosphatidylinositol phospholipase D1 | 2.77 |
| SLG775 pattern | |||
| C89959 | SSG694 | Dictyostelium thioredoxin 3 | 7.42 |
| AU074665 | SSL481 | Dictyostelium hypothetical protein AAL92 | 5.9 |
| AU060648 | SLK756 | Dictyostelium prestalk D11 protein | 4.86 |
| C91531 | SSK395 | Adducin 1 (alpha) (1e-35) | 4.29 |
| AU073534 | SSH476 | Dictyostelium hypothetical protein AAL92191.1 | 2.84 |
| AU061959 | SLG775 | Dictyostelium cystein-proteinase CPRB | 2.82 |
| SLH659 pattern | |||
| AU060263 | SLA769 | Dictyostelium hypothetical protein AAL92643.1 (GP64 homolog) | 10.9 |
| AU061762 | SLF774 | Dictyostelium β-glucosidase | 7.29 |
| AU037168 | SSB559 | Unknown | 7.22 |
| AU073811 | SSI424 | Unknown | 6.56 |
| AU038606 | SSL238 | Unknown | 6.28 |
| AU073596 | SSH630 | Dictyostelium hypothetical protein AAL93052.1 | 5.54 |
| AU062115 | SLH659 | δ-1-Pyrroline-5-carboxylate dehydrogenase (2e-66) | 5.36 |
| C93985 | SSC889 | Dictyostelium thioredoxin 2 | 3.92 |
| AU073683 | SSH823 | Unknown | 3.8 |
| AU074824 | SSM175 | Dictyostelium myosin light-chain kinase (MLCKA) (=SSJ249) | 3.32 |
| C91391 | SSK159 | Dictyostelium hypothetical protein AAL92274.1 (1e-15) (=SSB159) | 3.24 |
| AU072378 | SSE356 | Dictyostelium lysozyme | 2.96 |
| AU074364 | SSK495 | Potassium efflux system KEFA (8.7) | 2.78 |
| AU074982 | SSM642 | Retinal pigment epithelial protein (0.005) | 2.63 |
| AU071713 | SSC340 | Unknown | 2.39 |
| AU075086 | SSA210 | Unknown | 2.23 |
| AU073353 | SSG754 | Dictyostelium calcium/binding protein 1 | 2.17 |
| C90552 | SSI714 | Dictyostelium phosphatidylinositol transfer protein 2 | 2.01 |
| PstAO genes | |||
| SSH475 pattern | |||
| C92972 | SSF295 | Unknown | 9.6 |
| C84222 | SSB337 | Unknown | 9.2 |
| AU062233 | SLI566 | Homologous to yeast ACR1/protein homolog (7e-19) | 7.4/PICK> |
| C90923 | SSJ314 | Unknown | 5.8 |
| C91268 | SSJ728 | Unknown (=SSF823) | 5.49 |
| AU038875 | SSL584 | Unknown | 5.04 |
| AU074602 | SSL349 | Unknown | 4.76 |
| AU073356 | SSG767 | Unknown | 4.46 |
| C90189 | SSI228 | Extracellular endoglucanase (0.001) | 4.26 |
| AU074795 | SSL886 | Unknown | 4.1 |
| C92856 | SSF509 | Unknown | 4.01 |
| AU074211 | SSJ814 | Unknown (=SSM194) | 3.99 |
| AU073533 | SSH475 | Unknown | 3.23 |
| C92333 | SSD873 | Acetoin dehydrogenase (8e-14) | 3.2 |
| AU038842 | SSL550 | Unknown | 3.05 |
| AU062252 | SLI716 | Unknown | 2.89 |
| AU073109 | SSG159 | Unknown | 2.75 |
| AU060133 | SLA387 | β-1,4-endoglucanase (7e-19) (=SFE338) | 2.66 |
| C89985 | SSG805 | Unknown | 2.57 |
| AU061483 | SLE410 | Dictyostelium RASD | 2.49 |
| AU074481 | SSK868 | Dictyostelium extracellular matrix protein homolog (2e-20) | 2.37 |
| C91092 | SSJ538 | Dictyostelium P17 cyclic AMP-regulated protein | 2.3 |
| AU061713 | SLF535 | Dictyostelium kielin | 2.25 |
| AU071694 | SSC290 | Dictyostelium ECMB homolog (5e-26) | 2.22 |
| AU038846 | SSL554 | Cerebroside sulfate activator (4e-15) (=SSL531) | 2.2 |
| AU073407 | SSH123 | Dictyostelium hypothetical protein AAL92997.1 | 2.17 |
| SLE474 pattern | |||
| AU037288 | SSB721 | Dictyostelium hypothetical protein AAL92651.1 | 4.14 |
| AU035004 | SLE474 | Dictyostelium prestalk ECMA protein | 3.79 |
| AU060824 | SLB609 | Acid phosphatase (9e-17) (=VFB675) | 3.17 |
| C94063 | SSG357 | Dictyostelium CAR1; cyclic AMP Receptor 1 | 3.16 |
| AU062087 | SLH511 | Dictyostelium DOCA | 3.04 |
| AU071989 | SSD123 | Dictyostelium OSBPa | 2.7 |
| SSA854 pattern | |||
| AU072779 | SSA854 | Unknown | 5.85 |
| AU039124 | SSM416 | Unknown (=SSL853) | 5.08 |
| AU074011 | SSJ246 | Unknown | 3.88 |
| AU071496 | SSB661 | Unknown | 2.64 |
| AU060119 | SLA364 | Dictyostelium ACTIN5 | 2.41 |
| C90964 | SSJ359 | Unknown | 2.22 |
| Late prestalk genes | |||
| SSE634 pattern | |||
| C84694 | SSE634 | Phosphatidylglycerol/phosphatidylinositol transferase (1e-13) | 5 |
| AU074273 | SSK196 | Homolog of Neurospora glutamine dehydrogenase (2e-81) | 4.84 |
| AU060554 | SLK384 | Unknown | 3.8 |
| AU062051 | SLH367 | Glutamine synthase (4e-33) | 3.63 |
| AU071922 | SSC746 | Dictyostelium extracellular matrix protein | 3.6 |
| C93190 | SSM756 | Unknown | 2.92 |
| AU072597 | SSA348 | Dictyostelium hypothetical protein AAB06761.1 (2e-08) | 2.52 |
| AU060917 | SLC233 | Dictyostelium phosphoenolpyruvate carboxykinase | 2.46 |
| C89827 | SSG207 | Caffeic acid O-methyltransferase (7e-14) | 2.26 |
| C89911 | SSG614 | Dictyostelium δ5 fatty acid desaturase | 2.17 |
| AU034900 | SLE660 | Dictyostelium isocitrate lyase | 2 |
| AU071371 | SSB338 | Unknown | 4.84 |
| AU062192 | SLI252 | Dictyostelium DP87 precursor (4e-04) | 4.03 |
| AU052407 | SLD267 | Putative allantoinase precursor (5e-29) | 2.7 |
| SSG721 pattern | |||
| AU061693 | SLF442 | Dictyostelium malate synthase | 3.75 |
| C92120 | SSD115 | Homologous to Plasmodium falciparum hypothetical protein PFC0486 homolog (2e-10) | 3.13 |
| AU073340 | SSG721 | Oxalate/formate antiporter (3e-14) | 3.13 |
| AU053631 | SLJ273 | Dictyostelium cytosolic aldolase | 3.13 |
| AU037927 | SSE462 | Haloacid dehalogenase-like hydrolase (7e-14) | 2.64 |
| AU062141 | SLH752 | Dictyostelium hypothetical protein (4e-19) | 2.54 |
| AU062057 | SLH405 | Putative polyketide synthase module protein (1e-49) | 2.51 |
| AU072355 | SSE273 | Acyl coenzyme A oxidase (4e-08) | 2.47 |
| AU062008 | SLH173 | Dictyostelium Na/K-ATPase alpha4 (IonA) | 2.32 |
a
cDNA sequences were assigned to open reading frames found in the Dictyostelium genomic databases. Their protein products were compared to GenBank entries, and the most significant match is given, with the associated E value in parentheses. No E value is given for identity to established Dictyostelium proteins.