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. 2003 Jun;2(3):560–568. doi: 10.1128/EC.2.3.560-568.2003

FIG. 5.

FIG. 5.

Downregulation of TbRBP38 expression in procyclic T. brucei by inducible RNAi. RNAi was induced by the addition of 1 mg of tetracycline/liter. (A) Cumulative growth curve with or without tetracycline. Cell growth is expressed as the number of cell divisions. (B) Analysis of TbRBP38 downregulation in induced and mock-treated cells. A total of 20 μg of total protein was loaded per lane and then analyzed with anti-LtRBP38 antisera. p70 was used as a loading control. Next, 35 μg of RNA per time point was analyzed by primer extension as described in Materials and Methods. Calmodulin was used as a loading control.