TABLE 2.

Probes used for FISH and the corresponding hybridization and washing conditions

Probe	Sequence (5′→3′)	Target organisms	Conca		Competitor	Reference
			Formamide (%)	NaCl (M)
EUB338	GCTGCCTCCCGTAGGAGT	Bacteria	0	0.9		1
EUB338II	GCAGCCACCCGTAGGTGT	Bacteria	0	0.9		4
EUB338III	GCTGCCACCCGTAGGTGT	Bacteria	0	0.9		4
NEU	CCCCTCTGCTGCACTCTA	Halophilic and halotolerant members of the genus Nitrosomonas	40	0.056	CTE	41
Ntspa662	GGAATTCCGCGCTCCTCT	Nitrospira genus	35	0.08	CNtspa662	5
NmII	TTAAGACACGTTCCGATGTA	Many members of the Nitrosomonas communis sublineage	25	0.159		26
Nsv443	CCGTGACCGTTTCGTTCCG	Most Nitrosospira spp.	30	0.112		23
Nso1225	CGCCATTGTATTACGTGTGA	Almost all ammonia-oxidizing β-Proteobacteria	35	0.08		23
Nso190	CGATCCCCTGCTTTTCTCC	Many but not all ammonia-oxidizing β-Proteobacteria	50	0.028		23
Nmo218	CGGCCGCTCCAAAAGCAT	Many members of the Nitrosomonas oligotropha sublineage	35	0.08		10
NmV	TCCTCAGAGACTACGCGG	Nitrosococcus mobilis	35	0.08		26
Nit3	CCTGTGCTCCATGCTCCG	Nitrobacter	40	0.056	CNIT3	42
CNIT3	CCTGTGCTCCAGGCTCCG	Competitor to Nit3				42
CTE	TTCCATCCCCCTCTGCCG	Competitor to NEU
CNtspa662	GGAATTCCGCTCTCCTCT	Competitor to Ntspa662

^aConcentrations presented as percentage of formamide in hybridization buffer or molar concentration of NaCl in wash buffer.