Figure 5.

Galectin-3 protects B cells from Fas-induced apoptosis. A: Flow cytometry analysis of annexin V and propidium iodide-stained galectin-3-positive BCBL-1 PEL cells transfected with a galectin-3C-expressing or vector control construct (left four panels) and galectin-3-negative Raji BL cells transfected with a galectin-3-expressing or vector control construct (right four panels) before or after treatment with anti-Fas antibody for 24 hours. B: Summary of cell death data, shown as the percent change in apoptosis between untreated and anti-Fas-treated cells when stably transfected with galectin-3, galectin-3C, and vector control expression constructs. Data are mean ± SD of triplicate samples and is representative of three independent experiments.