Figure 2.

A: Total RNA, protein, and genomic DNA were extracted from each cell line, and Northern blot (N.B.) or Western blot analysis (W.B.) was performed with probe or antibody for ARK5 or β-actin. Southern blot analysis (S.B.) was also performed with probe for ARK5 or PERK. B: Strong association between ARK5 expression and tumor cell invasion activity. Each cell line was seeded on a matrigel-coated invasion chamber (5 × 10⁴ cells/chamber), and cells that had translocated to the lower chamber were counted after 48 hours. Results are shown as means of three experiments, and the bars represent SE values.