Figure 6.

Temporal change of immunofluorescence for E-selectin on HUVECs after incubation with placental-unconditioned medium (a–d), conditioned medium from tissues kept under normoxia (e–h), or conditioned medium from tissues subjected to H/R (i–l). Immunofluorescence for E-selectin was first detectable after 6 hours of incubation and became more apparent at 24 hours in cells grown in media containing placental-conditioned media from tissues subjected to H/R or kept normoxic throughout. In contrast, placental-unconditioned media caused only a few cells to express E-selectin at 24 hours.