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. 2003 Jun;69(6):3681–3685. doi: 10.1128/AEM.69.6.3681-3685.2003

FIG. 1.

FIG. 1.

 (a) Cross sensitivity study. DPC3147, DPC5552, and DPC5552pMRC01 were grown as culture spots (10 μl) on LM17 agar plates and overlaid with each of DPC3147, DPC5552, and DPC5552pMRC01. The appearance of zones indicates inhibition of the indicator due to bacteriocin production by the producer. (b) Identification of a portion of the lacticin 481 structural gene, lctA, by PCR using primers designed to amplify a 720-bp fragment of the gene. A linear 100-bp DNA ladder (lane 1) and a 1-kb DNA ladder (lane 18) were used as size markers. Lanes: 2, positive control (L. lactis DPC5552 genomic DNA as template); 3, positive control (L. lactis CNRZ481 genomic DNA as template); 4, negative control (L. lactis MG1614 genomic DNA as template); 5, negative control (L. lactis MGpMRC01 genomic DNA as template); 6 to 17, putative transconjugants. A band corresponding to a fragment of 720 bp is evident in lanes 2, 3, and 6 to 17, indicating that all these transconjugants contain the gene for lacticin 481 biosynthesis. (c) Identification of a portion of the lacticin 3147 immunity gene, ltnI, by PCR using primers designed to amplify a 923-bp fragment of the gene. A 1-kb DNA ladder (lane 1) and a linear 100-bp DNA ladder (lane 18) were used as size markers. Lanes: 2, positive control (L. lactis MGpMRC01 genomic DNA as template); 3, negative control (L. lactis MG1614 genomic DNA as template); 4, negative control (L. lactis DPC5552 genomic DNA as template); 5, negative control (L. lactis CNRZ481 genomic DNA as template); 6 to 17, putative transconjugants. A band corresponding to a fragment of 923 bp is evident in lanes 2 and 6 to 17, indicating that all these transconjugants contain the gene for lacticin 3147 immunity.