TABLE 2.
Intracellular concentrations of DnaK in E. coli ATCC 25922, after incubation in BHI broth at various temperaturesa
| Temp (°C) | No. of DnaK molecules/cellb | Ratio of DnaK to total proteinsc (%)
|
|
|---|---|---|---|
| Exptl value | Value cited in literatured | ||
| 30 | 18,767 ± 3,044 | 0.63 ± 0.11 | 0.66 |
| 37 | 38,500 ± 6,056 | 0.98 ± 0.07 | 1.00 |
| 42 | 103,867 ± 13,735 | 1.60 ± 0.32 | 1.42 |
Cells were grown until an OD600 of 0.5 was reached. The experiments were repeated three times.
The concentrations per cell were calculated with the number of cells present before the treatment.
Total proteins were evaluated after sonification.
Values are cited by Herendeen et al. (8).