TABLE 3.

Intracellular DnaK concentration and cell counts of E. coli ATCC 25922 cells after 0, 12, and 24 h of recovery following heat treatments^a

Timeb (h)	Result at:
	F7010 = 3 min (55°C, 105 min)				F7010 = 5 min (55°C, 150 min)
	DnaK concn (no. of molecules/cell)	Cell count			DnaK concn (no. of molecules/cell)	Cell count
		Agar platesc (log10 CFU/ml)	Fluorescence			Agar plates (log10 CFU/ml)	Fluorescence
			Live cells	Dead cells			Live cells	Dead cells
0	12,347 ± 2,363	<0.7	79	921	<1,500	<0.7	0	1,000
12	76,786 ± 25,230	4.57	90	910	<1,500	<0.7	0	1,000
24	56,443 ± 20,417	8.29	577	423	<1,500	<0.7	0	1,000

^aThe experiment was repeated three and two times for F₇₀¹⁰ values of 3 and 5 min, respectively.

^bRecuperation time at 37°C after heat treatment.

^cA volume of 200 μl spread on five plates for a detection limit of <0.7 log₁₀ CFU/ml.