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Use of pEM1, containing a PmpfA-lacZ fusion, to examine the influence of medium composition and growth phase on expression of conjugal transfer genes in P. putida Cg1. Expression of mpfA during growth in three different liquid media (A) and after 1 day of growth on agar (B). Labels for media appear in panel B. Each treatment was replicated three times. Error bars show standard deviation. β-galactosidase activities are expressed in Miller units. OD600, optical density at 600 nm.
