TABLE 2.

Primers used in this study to amplify and analyze portions of a 26.8-kb region of naphthalene catabolic plasmids pDTG1 and pCg1^a

Primer name	Sequence (5′→3′)	Corresponding region of 26.886 kb
Primers used for cloning and sequencing
mpfA-Fb	CCC GGT ACC CAA ACC CTG CAA AGC GTA ATC	21145-21125
mpfA-Rb	CCC GTC GAC GGC CAT GCT CTT CAA ACC ACT	20972-20992
orf25-F	ATG CAG AGC CTA GAC AAA AGA G	21521-21500
mpfB-R	TCA ACG AGG CGC ATA ACT TCT	20474-20494
parA-Fb	GGG GGT ACC CAT ATA TAG GGT TTA CTT TTC	22102-22082
mpfA-R2b	CCC GTC GAC GTC CAA CTT TGG AAG TTT	21205-21222
tra-Fb	CGC GAA TTC GTC AAA GTC TCA ATA CAC AAC ACG	2683-2706
traB-Rb	CGC GGT ACC AAT CGA CTT CTC TTT TAT GC	3316-3297
Primers used for RFLP analysis
493F	CGC GTG CCG AAG TGG TC	493-509
1272R	TGG TCG GGC AGA TGA TGG AAG ATT	1272-1249
1629F	TTG TTG AAG ACC ACC AGA ATC C	1629-1650
2437R	TGA GTC AAA GGC AGC GAA AAT ACG	2437-2414
4122F	GGA CTC CGA GGG CTA CTT	4122-4138
5108R	TCC GAC GAT TAA CTA TTT GAC	5108-5088
6779F	CGA CAG AAG AAC CAG GAA CTA AAA	6779-6802
7510R	TGT AAG ACC TTG GGC GCT GTG GAC	7510-7487
9422F	TCT TTG GCC ATG TCC TAA TCC TCT	9422-9445
10591R	GCT CGC GCT ATG GTT TCT AAG TTG	10591-10568
12475F	ACG CGG CCA GGT TTA CGA ATA CA	12475-12497
13114R	GCG CGG GTG CCT CCA ACT CC	13114-13095
17460F	GTG TTG ACC AGT TCC TCG TAA T	17460-17481
18239R	TCA TGC AGA TCC GCC GTA AAA	18239-18219
19699F	GCT CAG CTC GCA GTA TTC CT	19699-19718
20391R	GAG TTG GTG GTC AGC GAG ATG	20391-20371

^aNucleotide positions 1 and 26886 correspond to the extreme left and right, respectively, of Fig. 2. The mpf gene cluster (Fig. 2) is bounded by positions 8794 and 21232.

^bRestriction enzyme sites, used for cloning purposes, are underlined; when restriction enzyme sites were inserted into a primer, three additional nucleotides were added to the 5′ end.