TABLE 4.

Influence of mating time, medium, and solid surface on conjugal transfer frequencies of pCg1 between P. putida Cg1 (host) and P. putida KT2440-Tc (recipient)

Donor and recipient density in mating mix		Mating duration (h)	Donor medium	Conjugation frequencya
Donor	Recipient			Solid	Liquid
1.5 × 108 (1.30)b	2.7 × 108 (1.50)	1	LB	1.5 × 10−6	BDc
3.6 × 108 (0.98)	3.8 × 108 (1.30)	3	LB	2.7 × 10−7	1.3 × 10−9
3.2 × 107 (0.61)	7.4 × 107 (0.90)	3	LB	4.4 × 10−5	4.6 × 10−7
2.3 × 108 (1.10)	3.8 × 108 (1.30)	6	LB	1.3 × 10−4	3.9 × 10−6
1.9 × 108 (0.90)	1.9 × 109 (0.70)	24	LB	1.9 × 10−4	1.1 × 10−5
2.0 × 107 (1.10)	2.7 × 108 (1.50)	1	MSB-G	BD	BD
1.8 × 108 (0.99)	5.8 × 108 (1.05)	3	MSB-G	BD	BD
9.8 × 108 (0.95)	6.0 × 106 (0.70)	6	MSB-G	3.7 × 10−7	BD
2.4 × 108 (1.05)	6.0 × 106 (0.70)	6	MSB-N	3.9 × 10−6	BD

^aFrequencies are expressed as the number of transconjugants per donor cell (transconjugant/donor ratio).

^bMeasurement of optical density at 600 nm is given in parentheses.

^cBD, below detection limit.