TABLE 2.
Comparison of PCR-ELISA with culture method for detecting S. enterica, C. coli, and C. jejuni on poultry or in the poultry production environment
| Sp. detected | No. of samples with result/total no.
|
Overall agreementc | |||
|---|---|---|---|---|---|
| PCR-ELISA+, culture+ | PCR-ELISA+, culture− | PCR-ELISA−, culture+ | PCR-ELISA−, culture− | ||
| Campylobactera | 31/32 | 0/32 | 1/32 | 0/32 | 31/32 (0.97) |
| Salmonella (direct)a | 20/120 | 6/120 | 10/120 | 84/120 | 104/120 (0.87) |
| Salmonella (enrichment)b | 29/60 | 4/60 | 1/60 | 26/60 | 55/60 (0.92) |
a
Detection of pathogen in chicken carcass rinses.
b
Detection of S. enterica in samples following overnight enrichment culture in TBG.
c
Number of true positives plus true negatives divided by the total sample number.