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. 2003 Jun;69(6):3580–3592. doi: 10.1128/AEM.69.6.3580-3592.2003

TABLE 2.

FISH-direct count analysis of microbial populations within Finna

Type of analysis Z1
Z2
Z3
Z4
Cell concn (cells/g [dry wt] of sulfide)b % of probed cellsc Cell concn (cells/g [dry wt] of sulfide)b % of probed cellsc Cell concn (cells/g [dry wt] of sulfide)b % of probed cellsc Cell concn (cells/g [dry wt] of sulfide)b % of probed cellsc
DAPI (1.9 ± 0.2) × 106 (1.7 ± 0.2) × 108 (1.9 ± 0.3) × 107 (2.1 ± 1.2) × 105
ARCH915 (6.3 ± 1.1) × 105 33 (1.1 ± 0.3) × 108 65 (1.2 ± 0.1) × 107 63 (4.8 ± 2.7) × 104 23
EUB338 (1.0 ± 0.3) × 106 53 (4.4 ± 1.3) × 107 26 (3.8 ± 1.1) × 105 2 ND 0
EUK502 (3.8 ± 1.2) × 104 2 NDd 0 ND 0 ND 0
CREN499 (1.7 ± 0.3) × 105 9 (5.8 ± 0.8) × 107 34 (8.3 ± 1.2) × 106 44 (2.8 ± 0.4) × 104 11
EURY498 (3.0 ± 0.9) × 105 16 (2.5 ± 0.6) × 107 15 (2.8 ± 0.8) × 106 15 (8.3 ± 2.0) × 103 3
TC589 (1.6 ± 1.6) × 104 <1 (3.0 ± 1.0) × 106 2 (3.6 ± 2.4) × 105 2 (1.9 ± 1.9) × 103 <1
MC688 + MC1109 (2.3 ± 1.2) × 104 <1 (6.0 ± 1.2) × 106 4 (9.6 ± 3.6) × 105 5 (1.7 ± 1.7) × 103 <1
Lipid 3.4 × 106e 1.7 × 108e ND ND
a

The predominant mineralogy of the zones was as follows: Z1, pyrite and ZnS (wurtzite and sphalerite) with or without marcasite and with or without iron oxyhydroxides; Z2, pyrite, ZnS (wurtzite and sphalerite), and amorphous silica with or without barite and with or without clay; Z3, pyrite and ZnS (wurtzite and sphalerite) with or without chalcopyrite and with or without anhydrite; and Z4, chalcopyrite with or without anhydrite and with or without ZnS (wurtzite and sphalerite). The average porosities of the mineralized zones based on petrological thin sections examined at a magnification of × 50 (500-μm field of view) were as follows: Z1, 30 to 40%; Z2, 15 to 20%; Z3, 20 to 25%; and Z4, 5 to 10%.

b

For all data except the lipid analyses, the data are means ± 95% confidence intervals for four transects. The limit of detection was 1.6 × 103 cells/g.

c

Percentage of probed cells in the DAPI-stained total population.

d

ND, not detected.

e

Lipid-derived prokaryotic cell density determined by using the conversion factor of Balkwill et al. (7). The limit of detection was 1.25 × 106 cells/g.

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