TABLE 2.
FISH-direct count analysis of microbial populations within Finna
| Type of analysis | Z1
|
Z2
|
Z3
|
Z4
|
||||
|---|---|---|---|---|---|---|---|---|
| Cell concn (cells/g [dry wt] of sulfide)b | % of probed cellsc | Cell concn (cells/g [dry wt] of sulfide)b | % of probed cellsc | Cell concn (cells/g [dry wt] of sulfide)b | % of probed cellsc | Cell concn (cells/g [dry wt] of sulfide)b | % of probed cellsc | |
| DAPI | (1.9 ± 0.2) × 106 | (1.7 ± 0.2) × 108 | (1.9 ± 0.3) × 107 | (2.1 ± 1.2) × 105 | ||||
| ARCH915 | (6.3 ± 1.1) × 105 | 33 | (1.1 ± 0.3) × 108 | 65 | (1.2 ± 0.1) × 107 | 63 | (4.8 ± 2.7) × 104 | 23 |
| EUB338 | (1.0 ± 0.3) × 106 | 53 | (4.4 ± 1.3) × 107 | 26 | (3.8 ± 1.1) × 105 | 2 | ND | 0 |
| EUK502 | (3.8 ± 1.2) × 104 | 2 | NDd | 0 | ND | 0 | ND | 0 |
| CREN499 | (1.7 ± 0.3) × 105 | 9 | (5.8 ± 0.8) × 107 | 34 | (8.3 ± 1.2) × 106 | 44 | (2.8 ± 0.4) × 104 | 11 |
| EURY498 | (3.0 ± 0.9) × 105 | 16 | (2.5 ± 0.6) × 107 | 15 | (2.8 ± 0.8) × 106 | 15 | (8.3 ± 2.0) × 103 | 3 |
| TC589 | (1.6 ± 1.6) × 104 | <1 | (3.0 ± 1.0) × 106 | 2 | (3.6 ± 2.4) × 105 | 2 | (1.9 ± 1.9) × 103 | <1 |
| MC688 + MC1109 | (2.3 ± 1.2) × 104 | <1 | (6.0 ± 1.2) × 106 | 4 | (9.6 ± 3.6) × 105 | 5 | (1.7 ± 1.7) × 103 | <1 |
| Lipid | 3.4 × 106e | 1.7 × 108e | ND | ND | ||||
The predominant mineralogy of the zones was as follows: Z1, pyrite and ZnS (wurtzite and sphalerite) with or without marcasite and with or without iron oxyhydroxides; Z2, pyrite, ZnS (wurtzite and sphalerite), and amorphous silica with or without barite and with or without clay; Z3, pyrite and ZnS (wurtzite and sphalerite) with or without chalcopyrite and with or without anhydrite; and Z4, chalcopyrite with or without anhydrite and with or without ZnS (wurtzite and sphalerite). The average porosities of the mineralized zones based on petrological thin sections examined at a magnification of × 50 (500-μm field of view) were as follows: Z1, 30 to 40%; Z2, 15 to 20%; Z3, 20 to 25%; and Z4, 5 to 10%.
For all data except the lipid analyses, the data are means ± 95% confidence intervals for four transects. The limit of detection was 1.6 × 103 cells/g.
Percentage of probed cells in the DAPI-stained total population.
ND, not detected.
Lipid-derived prokaryotic cell density determined by using the conversion factor of Balkwill et al. (7). The limit of detection was 1.25 × 106 cells/g.