TABLE 4.
Distribution of T-RFLP fragments across transects at the Carpenter site
| Location | No. of positive transectsa with restriction enzyme.
|
||||||
|---|---|---|---|---|---|---|---|
| TaqI (283 bp) |
CfoI
|
AluI
|
|||||
| 66 bp | 68 bp | 100 bp | 135 bp | 392 bp | 491 bp | ||
| M1 | 3 | 3 | 0 | 0 | 2 | 3 | 1 |
| M2 | 3 | 3 | 0 | 0 | 1 | 3 | 0 |
| M3 | 3 | 3 | 2 | 0 | 2 | 3 | 2 |
| M/F | 3 | 3 | 0 | 0 | 3 | 3 | 2 |
| F/M | 3 | 3 | 0 | 2 | 2 | 3 | 2 |
| F1b | 2 | 2 | 1 | 1 | 1 | 2 | 0 |
| F2 | 3 | 3 | 0 | 0 | 0 | 3 | 0 |
| F3 | 3 | 3 | 1 | 0 | 1 | 3 | 1 |
| Total | 23 | 23 | 4 | 3 | 12 | 23 | 8 |
a
There are three transects across the site; therefore, three of each specific location per site is the maximum number per location.
b
PCR products of amoA were not successfully produced from one F1 soil sample.