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Purification of lawsone reductase activity from E. coli K-12a
| Purification step | Vol (ml) | Total protein (mg) | Sp. act (U/mg) | Total activity (U) | Recovery (%) | Purification (fold) |
|---|---|---|---|---|---|---|
| Crude extract | 23 | 308 | 0.058 | 18.0 | 100 | 1 |
| Q-Sepharose | 5 | 8.4 | 1.75 | 14.6 | 81 | 30 |
| Mono-P | 6 | 0.6 | 9.9 | 6.25 | 35 | 170 |
| Mono-Q | 1 | 0.2 | 29 | 5.8 | 32 | 500 |
Experimental details are given in Materials and Methods.
