TABLE 2.
Oligonucleotides used for DNA amplification by PCR
| Primer | Sequencea |
|---|---|
| Fol-F | CATACCACTTCTTTTTCGATTTGTAAAGG |
| Hom-R | CGATCCCGGGAAGCCCTGTGCCAACTGTCC |
| FolKE-F | CATGCCATGGGGCAAACAACTTATTTAAGCATGG |
| FolKE-R | GGGGTACCGATTCTTGATTAAGTTCTAAG |
| FolC-F | GAAGAGGTACCAGAAGAGTTTAAAAAGTATTATCG |
| FolC-R | TCTCTAGACTACTTTTCTTTTTTCAAAAATTCACG |
| FolP-F | GAATGGTACCTTTAGGAGGTCTTTTATGAAAATCTTAGAAC |
| FolP-R | GAGAAATCAAATCCTCATTCTAGATTAAAATTCC |
| FolA-F | GGAATTCCATGGTTATTGGAATATGGGCAGAAG |
| FolA-R | GCCTCAAGCTTCATGGTTGTTTCACTTTTTC |
| FolA-ASF | GAGGGGTACCTATGATAATTGGAATATGG |
| FolA-ASR | GCCCAAAAATTGATTTTGCCATGGTTG |
| Pcon-F | GAAGATCTGTCGACCTGCAGTAGACAGTTTTTTTAATAAG |
| Pcon-R | CGGGATCCGCATGCCTTCTCCTAAATATTCAGTATTAA |
| TpepV-F | CGGGATCCTTATGAACTTGCAAAATAAG |
| TpepV-R | GAAGATCTCACCTCTATTTCTAGAATAAAG |
| FolKE2-F | ATACATGCATGCAAACAACTTATTTAAGCATGGG |
| FolKE2-R | ATACATGCATGCGATTCTTGATTAAGTTCTAAG |
a
Underlined nucleotides represent modifications with regard to the mature gene.