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. 2004 Apr;164(4):1417–1424. doi: 10.1016/S0002-9440(10)63228-2

Figure 4.

Figure 4

Western blot analysis for cyclin D1 and cyclin D3 on glomerular protein from PHN rats. A: There was an increase in cyclin D1 protein expression in glomerular protein from day 5 PHN animals (lane 2) compared to control (lane 1), and the increase was more pronounced in PHN rats given bFGF (lane 3). Lane 4 shows that cyclin D1 levels normalized by day 30 PHN. Cyclin D3 protein levels were not changed in PHN. B: Quantification of the Western blots further shows that cyclin D1 levels increase in PHN, however, cyclin D3 levels did not change significantly. C: Co-immunoprecipitating glomerular protein from PHN day 5 rats with an antibody to cdk4 showed a positive signal for cyclin D1 (lane 2), which was absent in glomerular protein from control rats (lane 1).