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. 2004 Apr;164(4):1183–1190. doi: 10.1016/S0002-9440(10)63206-3

Figure 1.

Figure 1

A: Schematic diagram of 24-kd FGF-2 and its truncated form ATE + 31. The three CTG translation initiation sites are shown at the ATE. The AUG translation initiation site represents the ATE of 18-kd FGF-2. B: The effects of 24-kd FGF-2 and ATE + 31 on MCF-7 cell migration in a Boyden Chamber assay (right panel) and cell proliferation (left panel) were tested. ATE + 31 at 3.3 × 10−10 M and 24-kd FGF-2 at 6.6 × 10−11 M were used and the cell migration rates in response to 10 ng/ml IGF-1 were measured. Uncoated filters were used in this study. Results are presented as a percentage of the migration rate of MCF-7 cells in the presence of 10 ng/ml IGF-1 alone and represent the average ± SD of at least three separate experiments. Dose response experiments are described in Reference 14. The effect of ATE + 31 and 24-kd FGF-2 on proliferation of MCF-7 cells was determined at the same concentrations by measuring the rate of thymidine incorporation. 24-kd FGF-2 stimulated thymidine incorporation 8- to 10-fold while ATE + 31 had no effect. Results are presented relative to the rate of thymidine incorporation in the absence of any growth factor (indicated by the horizontal line) and represent the average of two experiments.