TABLE 1.

Primers used to generate full-length or truncated prtB genes cloned in plasmid pNZ124.1

Primer	Sequence	pNZ124.1 derivative with full-length or truncated prtB genes	Proteinase expressed
2162a	5′-TTTTGTGGATCCTTAACTTCATAGCACG
2164	5′-ATATTATCTAGAATTGAATAGATTGCC	pMD112	PrtB
9049	5′-CTAGTCTAGACCAACTACCTTGCC	pMD116	PrtBδ99b
G059	5′-CATGTCTAGATTACGTTCGCCATTGCC	pMD142	PrtBδ168
G060	5′-CATGTCTAGAAGTCAGCTTAACTTCGC	pMD143	PrtBδ247
9048	5′-CTAGTCTAGACCATCCTTGACTGGC	pMD115	PrtBδ806

^aForward primer; all others are reverse primers.

^bThe numbers in the designations indicate the number of deleted amino acids at the carboxy end of the proteinase.