TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or phenotype | Reference or source |
|---|---|---|
| T. roseopersicina strains | ||
| BBS | Wild type | 2 |
| RM26 | BBS crtD::Km | This work |
| E. coli strains | ||
| XL1-Blue MRF′ | Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endAL supE44 thi-1 recA1 gyrA96, relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)] | Stratagene |
| S17-1(λpir) | 294 (recA pro res mod) Tpr Smr (pRP4-2-Tc::Mu-Km::Tn7) λpir | 8 |
| BL21(DE3) | E. coli B F−dcm ompT hsdS(rB− mB−) gal λ(DE3) | Novagen |
| Plasmids | ||
| pTnMod-OKm | Kmr; Tn5-based plasposon delivery plasmid with Kmr | 4 |
| pGEM T-Easy | Ampr; cloning vector | Promega |
| pBluescript SK(+) | Ampr; cloning vector | Stratagene |
| pBBRexSm2 | Smr; broad host range vector | B. Fodor unpublished |
| pRM261 | 3.5-kb BamHI fragment harboring the plasposon from RM26 | This work |
| pRM265 | 4.9-kb KpnI fragment harboring the plasposon from RM26 | This work |
| pRM268 | 18.8-kb XbaI fragment harboring the plasposon from RM26 | This work |
| pSOX | pBluescript KS(+); carries 1.2-kb SacI fragment of crtD from Rubrivivax gelatinosus | 18 |
| pSO24 | pBluescript KS(+); carries 1.8-kb SacI fragment of crtD-crtC from Rubrivivax gelatinosus | 18 |
| pRcrt3 | pBluescript SK(+); carries the ApaI-SacI fragment of promoterless crtD-crtC from Rubrivivax gelatinosus | This work |
| pRcrt4 | Derivative of pRcrt3; contains the promoter of crtD from T. roseopersicina | This work |
| pRcrt5 | pBBRexSm2 containing BamHI-KpnI fragment of pRcrt4 | This work |
| pTcrt3 | pBluescript SK(+); carries the wild-type BamHI-SacI fragment of the crtDC operon of T. roseopersicina | This work |
| pTcrt4 | pBBRexSm2 containing BamHI-SacI fragment of pTcrt4 | This work |
| pPHU235 | Broad-host-range lacZ fusion vector | 9 |
| pK18mobsacB | Kmr; sacB; RP4 oriT; ColE1 ori | 28 |
| pKlac2 | EcoRI-SalI fragment from pPHU235 in pK18mobsacB | This work |
| pCrtlac4 | pKlac2 with 1,067-bp PstI-XhoI fragment from pRM265 | This work |
| pCrtlac9 | pKlac2 with 1,067-bp PstI-XhoI fragment from pRM265 | This work |
| pET28::CrtJ | pET28 overexpression plasmid with crtJ gene | 23 |
| pCRTI | pGEM T-Easy; contains 444-bp fragment of crtI | This work |
| pPPSR | pGEM T-Easy; contains 929-bp fragment of ppsR | This work |