TABLE 2.

Description of the ORFs identified

Genea	Known or putative function of product	Length (aa)	nt (start-stop)	Top BLAST hits
				Gene product	% Identity/E value
bchB	Light-independent prochlorophyllide reductase b subunit	258	1-777	Rubrivivax gelatinusus BchB	72/e-107
bchH	Mg-protoporphyrin IX chelatase H subunit	1,245	752-4489b	Rubrivivax gelatinusus BchH	66/0.0
bchL	Light-independent prochlorophyllide reductase iron-sulfur ATP binding subunit	294	4513-5397	Rhodospirillum rubrum BchL	66/e-105
				Rubrivivax gelatinusus BchL	64/e-105
bchM	Mg-protoporphyrin methyltranserase	233	5397-6098	Rubrivivax gelatinusus BchM	60/9e-76
orf86	Hypothetical protein	86	6095-6355
puhA	Photosynthetic reaction center H subunit	255	6381-7148	Thermochromatium tepidum PuhA	72/e-108
				Rubrivivax gelatinusus PuhA	49/e-63
orf218(G)	Hypothetical membrane protein	218	7145-7801	Rubrivivax gelatinusus ORF227	46/e-43
orf139	Hypothetical protein	139	7853-8272	Rubrivivax gelatinusus ORF154	39/9e-18
orf312	Hypothetical membrane protein	312	8535-9473c	Rubrivivax gelatinusus ORF276	43/6e-58
bchE	Mg-protoporphyrin IX monomethylester oxidative cyclase subunit	551	9864-11519c	Heliobacillus mobilis BchE	70/0.0
hemN	O2-independent coproporphyrinogen III oxidase	453	11533-12894	Aquifex aeolicus HemN	38/6e-77
				Rubrivavax gelatinusus HemN	40/2e-76
bchJ (G)	4-Vinyl reductase	208	12870-13496b	Rhodobacter sphaeroides BchJ	42/e-35
orf543	Long-chain fatty acid CoA ligase	543	13330-14961b	Halobacterius sp. strain Lfl1	38/3e-52
orf495 (G)	o-Succinyl-benzoic acid CoA ligase	495	13474-14961b	MenE Listeria innocua	34/4e-40
crtC ⇐	Hydroxyneurosporene dehydrogenase	405	16294-15077b	Rubrivivax gelatinusus CrtC	55/e-92
crtD ⇐	Methoxyneurosporene dehydrogenase	498	17487-15991	Rubrivivax gelatinusus CrtD	54/e-150
crtE	Geranylgeranyl pyrophosphate synthase	288	17607-18473c	Rubrivivax gelatinusus CrtE	55/6e-88
crtF (G)	Hydroxyneurosporene methyltransferase	371	18868-19982c	Rubrivivax gelatinusus CrtF	49/e-89
bchC (T)	2-α-Hydroxyethyl bacteriochlorophyllide oxidase	317	20092-21045	Rubrivivax gelatinusus BchC	61/e-106
bchX	Bacteriochlorophyllide reductase subunit	282	20871-21710	Bradyrhizobium sp. BchX	74/6e-79
		225	21042-21710	Rubrivivax gelatinusus BchX	73/2e-76

^aG and T, GTG and TTG start codons, respectively. The arrows indicate the inverse orientation of the ORFs.

^bOverlaps with preceding ORF by more than 10 bp.

^cGap longer than 50 bp.