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Expression of INP-MerR in different cellular fractions. Total cell proteins were separated by SDS-PAGE (12.5% [wt/vol] polyacrylamide) and transferred to a nitrocellulose membrane. Western blot analysis with anti-INP sera was performed at a 1:3,000 dilution. Lanes 1, 2, and 3 represent the total protein, soluble fraction, and membrane fraction of JM109/pUNIM. Lane 4 represents the total protein of JM109/pUC18Not. The desired fusion proteins are marked with an arrow. MW, molecular mass.
