FIG. 6.
(A) Insertion of a kanamycin resistance cassette into an EcoRV site of sll0088 that had been cloned as a HindIII fragment. Arrows show the directions of transcription. (B) Southern blot of various transformants probed with the shaded area shown in panel A. All transformed lines were taken through five serial platings under photoautotrophic growth selection conditions. Lanes 1 to 4 represent four independent cell lines of C14SPsaC transformed with psll0088K. Lane 5 contains 50 ng of DNA from wild-type cells. Lanes 6 to 8 show wild-type cell lines transformed with psll0088K and grown under heterotrophic, mixotrophic, and photoautotrophic conditions (46), respectively. The hybridization at 1.2 kb represents the 0.7-kb HindIII-EcoRV fragment of Synechocystis sp. strain PCC 6803 DNA and the 0.5-kb HincII-HindIII fragment of the inserted kanamycin resistance gene. The hybridization at 3.6 kb represents the wild-type sll0088 gene.