TABLE 1.
Physiological characterization of photoautotrophically grown cells of Synechocystis sp. strain PCC 6803a
| Cell typeb | Doubling time (h) | Chlorophyll/cellc | Whole-chain O2 evolutiond |
|---|---|---|---|
| WT-Gmr | 26.6 ± 3.2 | 1.65 ± 0.13 | 300.3 ± 42.4 |
| WT-sll0088K | 27.2 ± 5.3 | 2.96 ± 0.01 | 177.0 ± 21.1 |
| C14SPsaC-R18 | 34.1 ± 3.3 | 1.32 ± 0.06 | 298.8 ± 26.3 |
| C14SPsaC-R62 | 36.0 ± 3.4 | 1.40 ± 0.18 | 263.5 ± 44.7 |
| C14SPsaC-sll0088K | 37.7 ± 4.0 | 1.38 ± 0.14 | 142.4 ± 39.8 |
a
Results shown are averages and standard deviations from at least three independent cultures.
b
WT-Gmr, wild-type cells with an inserted gentamicin resistance gene; WT-sll0088K, wild-type merodiploid with a kanamycin resistance gene inserted in sll0088; C14SPsaC-R18 and C14SPsaC-R62, suppression mutant lines of C14SPsaC; C14SPsaC-sll0088K, C14S with a kanamycin resistance gene inserted into sll0088.
c
Shown as micrograms of chlorophyll per milliliter per unit of optical density at 730 nm at mid-log phase.
d
Shown as micromoles of O2 per milligram of chlorophyll per hour.