FIG. 4.
Primer extension analysis of put transcripts and sequence of putAP upstream region. (A) The transcription start sites were determined by primer extension of the RNA derived from V. vulnificus ATCC 29307 grown to log phase in LBS. Lanes G, A, T, and C represent the nucleotide sequencing ladders of pHJK002. The transcription start sites for P1 (C) and P2 (T) are indicated by white stars. (B) Transcription start sites are indicated by bent arrows. Possible promoters (−10 and −35) are shown underlined with continuous and broken lines for the promoters PputA and PputAP, respectively. Conserved nucleotide sequences for CRP binding (4) are indicated by capital letters. The ATG translation initiation codon and putative ribosome binding site (AGGA) are indicated in boldface type. ORF, open reading frame.