FIG. 5.

Dependence of proline dehydrogenase production of V. vulnificus on crp. Proline dehydrogenase activities (A) and relative amounts of the put transcripts (B and C) were determined for the wild-type (WT) strain and the isogenic crp mutant, KC74, as indicated. Complementation of the crp mutation by functional crp (pKC0004) is also presented. Samples removed from each culture grown to log phase in LBS were analyzed for proline dehydrogenase activity and put transcripts. Means ± SEM (error bars) are shown. PUTAP was used as a DNA probe, and the Northern blot is presented as described in the legend to Fig. 3. The relative amounts of the put transcripts in each band are expressed relative to the amount of the transcript A of KC74(pKC0004).