FIG. 9.
Effects of NaCl and glutamate on the activities of put promoters. Promoter activities were determined by primer extension of the RNA derived from the culture of ATCC 29307 grown to log phase in M9-N (None) and in M9-N supplemented with NaCl (0.6 M) or glutamate (20 mM) as indicated. Lanes G, A, T, and C contain the nucleotide sequencing ladders of pHJK002. The transcription start sites for PputA (P1) and PputAP (P2) are indicated by arrows.