TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Relevant characteristicsa	Reference or source
Strains
V. vulnificus
ATCC 29307	Clinical isolate; virulent	Laboratory collection
KC74	ATCC 29307 with crp::nptI; Kmr	15
HJK001	ATCC 29307 with putA::nptI; Kmr	19
HJK002	ATCC 29307 with putP::nptI; Kmr
E. coli
DH5α	supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1	Laboratory collection
SM10λpir	thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpir; Kmr; host for π-requiring plasmids; conjugal donor	Laboratory collection
Plasmids
pRK415	IncP ori; broad-host-range vector; oriT of RP4; Tcr	17
pHJK002	8.3-kb SalI fragment containing putAP cloned into pUC18; Apr	19
pKC0004	pRK415 with crp; Tcr	15

^aAp^r, ampicillin resistant; Km^r, kanamycin resistant; Tc^r, tetracycline resistant.