Figure 1.

In vitro treatment with LPS induces secretion of CyPA by HUVECs. A: CyPA (18 kd) was detected in the culture medium of HUVECs by Western blotting. LPS is an effective stimulator of CyPA secretion by ECs (6 hours). B: Expression of CyPA was stimulated by LPS (1 μg/ml) in serum-free media in a time-dependent manner, with maximal stimulation detected at 12 hours. C: Detection of CyPA in HUVECs by immunofluorescence (red fluorescent signal, CyPA; blue fluorescent signal, nuclei counterstained with Hoechst). Addition of monensin, an inhibitor of protein secretion, increases intracellular accumulation of CyPA. Scale bar, 5 μm.