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A: CyPA increases HUVEC invasiveness through a gelatin matrix (Transwell assay, VEGF 10 ng/ml). B: CyPA increases secretion and activation of gelatinolytic activity associated with matrix metalloproteinase-2 (sodium dodecyl sulfate-polyacrylamide gel electrophoresis gelatin zymography). No effects on secretion of the related gelatinase matrix metalloproteinase-9 were detected, suggesting matrix metalloproteinase-2 as the likely enhancer of endothelial invasiveness.
