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. 2004 May;164(5):1627–1633. doi: 10.1016/S0002-9440(10)63721-2

Figure 1.

Figure 1

Expression of CLDN 3 and 4 proteins in breast cancer cell lines, primary breast carcinoma, and normal mammary epithelium. A: Human primary breast carcinoma, normal mammary organoid tissue, and finite life span human mammary epithelial cells (HMEC) were homogenized and total protein was extracted. Western analysis was performed on equal amounts of protein from cell lysates using CLDN 3, 4, and β-actin antibodies. Significant differences in CLDN 3 (P = 0.008) and CLDN 4 (P = 0.046) expression levels between primary breast carcinomas and normal mammary epithelial cells were determined by Student’s t-test. B: CLDN 3 and 4 protein expression in human breast cancer cell lines. Western analysis was performed on equal amounts of protein from total cell lysates using CLDN 3, 4, and β-actin antibodies. C: The level of CLDN 3 and 4 expression in primary breast carcinomas, normal mammary organoids, and HMEC normalized to actin were determined by densitometric scanning of radiographical film. D: Immunohistochemical analysis was performed on paraffin embedded sections of human primary breast carcinoma tissues identified by an asterisk in A using CLDN 3 and 4 antibodies. CLDN 3 and 4 proteins in primary breast carcinoma tissue and adjacent normal mammary epithelium were visualized using DAB. Sections were counterstained with hematoxylin and visualized by light microscopy (422, ×200; 126 and 973, ×400).