Figure 3.

Sensitivity of human breast cancer cell line T47D to CPE-mediated cytolysis in vivo. Xenograft tumors were established in SCID mice using the human breast cancer cell line T47D. Tumors were allowed to grow to a size of approximately 100 mm³ and subsequently treated by intratumoral injection of 2 μg of CPE (A) or 10 μg of CPE (B) versus PBS alone on days 1, 3, 5, 7, 9, 11, and 13. Tumor volumes were measured on days 1, 7, and 14, after which animals were euthanized, tumors were removed, and tumor viability was determined by H&E and Ki67 staining (C). Each experiment is representative of 12 tumors. *P = 0.007 compared to volume on day 1 as determined by Student’s t-test.