TABLE 2.
Plasmids used in this study
| Plasmid | Description | Replicon | Selective marker | Reference |
|---|---|---|---|---|
| pET32a | High-level expression vector | pBR322 | Apr | 30 |
| pJB15 | Encodes PRD1 receptor | IncPα | Tcr | 24 |
| pLM2 | Encodes PRD1 receptor | IncPα | Kmr | 34 |
| pLysS | Produces small amount of phage T7 lysozyme that reduces the basal expression of genes cloned under the T7 promoter of pET32a vector | p15a | Cmr | 47 |
| pPR26 | PSU18 + nta 12984-13337 from PRD1 genome (ORF t) | p15a | Cmr | This study |
| pPR60 | PET32a + late promoter pR′ and gene S105 from lambda genome | pBR322 | Apr | This study |
| pPR61 | PET32a + nt 12984-13337 from PRD1 genome (ORF t) | pBR322 | Apr | This study |
| pPR62 | PSU18 + nt 4907-5294 from PRD1 sus715 genome (gene XXXI and ORF d) | p15a | Cmr | This study |
| pPR63 | PSU18 + nt 9427-9681 from PRD1 sus715 genome (ORF h) | p15a | Cmr | This study |
| pPR64 | PSU18 + nt 8332-8460 from PRD1 sus715 genome (ORF i) | p15a | Cmr | This study |
| pPR65 | PSU18 + nt 10044-10166 from PRD1 sus715 genome (ORF l) | p15a | Cmr | This study |
| pPR66 | PSU18 + nt 11090-11200 from PRD1 sus715 genome (ORF q) | p15a | Cmr | This study |
| pPR67 | PSU18 + nt 2415-3587 from PRD1 sus715 genome (ORF a, gene XV, ORF b) | p15a | Cmr | This study |
| pPR68 | PSU18 + nt 10617-10823 from PRD1 sus715 genome (ORF o) | p15a | Cmr | This study |
| pPR69 | PSU18 + nt 12984-13888 from PRD1 sus715 genome (ORFs t, u, v) | p15a | Cmr | This study |
| pPR70 | PSU18 + nt 12984-13337 from PRD1 sus715 genome (ORF t) | p15a | Cmr | This study |
| pPR71 | PSU18 + nt 12984-13337 from PRD1 sus712 genome (ORF t) | p15a | Cmr | This study |
| pPR72 | PSU19 + nt 12984-13337 from PRD1 sus711 genome (ORF t) | p15a | Cmr | This study |
| pPR73 | PSU19 + nt 12984-13337 from PRD1 sus714 genome (ORF t) | p15a | Cmr | This study |
| pPR74 | PSU19 + nt 12984-13337 from PRD1 sus716 genome (ORF t) | p15a | Cmr | This study |
| pPR75 | PSU18 + nt 12984-13337 from PRD1 sus718 genome (ORF t) | p15a | Cmr | This study |
| pPR76 | PSU18 + nt 12535-13692 from PRD1 H1 genome (ORF t + flanking sequences) | p15a | Cmr | This study |
| pPR77 | PSU18 + nt 12984-13337 from PRD1 H3 genome (ORF t) | p15a | Cmr | This study |
| pPR78 | PSU18 + nt 12535-13692 from PRD1 H3 genome (ORF t+ flanking sequences) | p15a | Cmr | This study |
| pPR79 | PSU19 + nt 12984-13337 from PRD1 H4 genome (ORF t) | p15a | Cmr | This study |
| pPR80 | PSU19 + nt 12535-13692 from PRD1 H4 genome (ORF t+ flanking sequences) | p15a | Cmr | This study |
| pPR81 | PSU18 + nt 12984-13337 from PRD1 H9 genome (ORF t) | p15a | Cmr | This study |
| pPR82 | PSU18 + nt 12535-13692 from PRD1 H9 genome (ORF t+ flanking sequences) | p15a | Cmr | This study |
| pS105 | Contains lambda late promoter pR′ and the lysis cassette, genes S105, R, Rz, and Rzl | pBR322 | Apr | 45 |
| pSU18/19b | Low-copy-number cloning vector | p15a | Cmr | 9 |
| pQ | Lambda gene Q cloned in pZS∗24 vector, containing the lac/ara hybrid promoter inducible by IPTG and arabinose | pSC101* | Kmr | 23 |
a
Nucleotides (nt) refer to positions in the recently updated PRD1 genomic sequence (GenBank accession no. M69077).
b
The only difference between vectors pSU18 and pSU19 is the opposite orientation of the polycloning site. Genes cloned into vector pSU18 can be expressed from the lac promoter. In the cloning of PRD1 ORFs, pSU19 was employed in those cases in which no correct constructs were obtained with pSU18.