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. 2003 Jul;185(13):3804–3812. doi: 10.1128/JB.185.13.3804-3812.2003

TABLE 1.

MICs of copper for E. coli strain GR1 mutant derivatives in LB medium containing copper chloride

Bacterial strain Relevant genotype Complementing gene in trans MIC (mM) of CuCl2a
GR1 ΔcueO None 3.25
GR15 ΔcueO ΔcusA::Cm None 1.5b
EC950 ΔcueO ΔcusB None 1.5b
EC951 ΔcueO ΔcusC None 1.75b
ECA067 ΔcueO ΔcusC ΔtolC::Kan None 1.75b
EC933 ΔcueO ΔcusF None 2.25
cusA mutant strains
    GR15(pECD738) ΔcueO ΔcusA::Cm cusA 2.75bc
    GR15(pECD739) ΔcueO ΔcusA::Cm cusA (M573I) 1.5bc
    GR15(pECD740) ΔcueO ΔcusA::Cm cusA (M623I) 1.25bc
    GR15(pECD741) ΔcueO ΔcusA::Cm cusA (M640I) 2.75bc
    GR15(pECD742) ΔcueO ΔcusA::Cm cusA (M672I) 1.25bc
    GR15(pECD743) ΔcueO ΔcusA::Cm cusA (M812I) 2.5bc
    GR15(pECD744) ΔcueO ΔcusA::Cm cusA (M738I) 2.75bc
    GR15(pECD745) ΔcueO ΔcusA::Cm cusA (M755I) 2.5bc
    GR15(pECD746) ΔcueO ΔcusA::Cm cusA (M792I) 2.75bc
    GR15(pECD747) ΔcueO ΔcusA::Cm cusA (M833I) 2.5bc
    GR15(pECD748) ΔcueO ΔcusA::Cm cusA (M875I-M878I-M881I) 2.5bc
    GR15(pECD768) ΔcueO ΔcusA::Cm cusA (D405N) 1.5c
    GR15(pECD769) ΔcueO ΔcusA::Cm cusA (E412Q) 1.5c
    GR15(pECD770) ΔcueO ΔcusA::Cm cusA (E412D) 2.5c
    GR15(pECD767) ΔcueO ΔcusA::Cm cusA (A399D) 1.75c
cusF mutant strains
    EC933(pECD735) ΔcueO ΔcusF cusF 2.75c
    EC933(pECD736) ΔcueO ΔcusF cusA (M69I-M71I) 2.25c
a

The MIC is defined as the lowest concentration at which no growth was observed. Strains were grown overnight and diluted 1:500 into fresh LB medium. After 2 h of growth at 37°C they were plated onto LB agar containing different concentrations (0.5 to 4.0 mM in steps of 0.25 mM) of CuCl2. The cusCFBA operon is fully expressed at copper concentrations above 100 μM (17). Growth was monitored after 16 h at 37°C. All experiments were done at least three times with identical results.

b

Colonies were small and mucoid at concentrations well below the MIC.

c

LB agar plates for complementation experiments contained anhydrotetracyline (200 μg/liter) to induce expression of complementing genes.