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. 2004 May;164(5):1547–1556. doi: 10.1016/s0002-9440(10)63713-3

Figure 5.

Figure 5

Double-fluorescence labeling of TNF-α/Kupffer cell, p-NF-κB/TNF-α, and superoxide/zinc ions in the liver. MT-KO mice were administrated with two doses of zinc sulfate at 5 mg of zinc ion/kg, followed by one dose of LPS at 4 mg/kg. Livers were removed at 1 hour after LPS challenge, and cryostat sections were cut at 5 μm. A: Co-localization of TNF-α/Kupffer cell (KC) and p-NF-κB/TNF-α by immunofluorescence staining. B: Simultaneous detection of superoxide (O2−) by dihydroethidine and zinc ions (Zn2+) by Zinquin. Arrowheads show the Kupffer cells and arrows show hepatocytes. Original magnifications, ×520.