FIG. 2.

(A) Swarming of the fliH null mutant with pET19b-based and pTrc99A-based plasmids encoding the proteins shown. V, vector. Plates were incubated for 4.5 h at 30°C. (B) Immunoblot, using anti-His antibody, of whole cells of the fliH null mutant transformed with plasmids encoding the proteins shown. (C) Immunoblot, using polyclonal anti-FlgD antibody, of the hook-capping protein FlgD in culture supernatants of the fliH mutant transformed with plasmids encoding the proteins indicated. Cellular levels of FlgD were the same in all cases (data not shown).