TABLE 3.
Quantitative analysis of nuclease, protease, and coagulase productiona
| Strain | Production
|
||
|---|---|---|---|
| Proteaseb | Nucleasec | Coagulased | |
| Becker | 2.48 ± 0.37 | 0.490 ± 0.181 | 100 ± 0 |
| CYL5614 | 0.53 ± 0.92 | 0.632 ± 0.180 | 14.8 ± 17.0 |
| CYL1040 | 3.33 ± 0.33 | 0.013 ± 0.023 | 100 ± 0 |
All data are averages (± standard deviations) of three independent experiments.
Protease production was analyzed by measuring depletion of 3 mg of azocasein/ml in 17 h, monitored by OD340.
Nuclease production was analyzed by measuring depletion of 5 mg of salmon sperm DNA/ml monitored by OD260.
Coagulase was measured by a tube coagulation test of threefold serial dilution samples. The numbers represent the highest dilution and are standardized by setting the activity of Becker as 100.