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Purification of MTMOIV enzyme
| Purification step | Total protein (mg) | Total activity (μmoles/min) | Specific activity (nmoles/min/mg of protein) | Purifi- cation (fold) |
|---|---|---|---|---|
| Cell-free extract | 120 | 0.088 | 0.7 | 1 |
| (NH4)2SO4 | 43.2 | 0.124 | 2.9 | 4.1 |
| Sepharose Blue 4 | 4.7 | 0.030 | 6.4 | 9.1 |
| Mono Q | 0.34 | 0.005 | 14.7 | 21 |
