TABLE 1.
E. coli strains and plasmids used in this study
| Strain or plasmid | Genotypea | Source or reference |
|---|---|---|
| E. coli | ||
| DH5α | Δ(argF lac) U196endA1recA1hsdR17(rK− mK+) supE44 thi-1 gyrA1 relA1 (F′ φ80 ΔlacZΔM15) | 11 |
| AA93 | Δfec aroB Δ(argF lac)U169 araD139 rspL150 relA1 deoC1 flbB5301 ptsF25 rbsR | 22 |
| SU202 | lexA71::Tn5 sulA211 sulA(op408/op+)::lacZ Δ(lacIPOZYA)169/F′ lacIqlacZΔM15::Tn9 | 5 |
| Plasmids | ||
| pSV66 | pHSG576 fecI fecR fecA | 12 |
| pMS604 | ori ColE1 TetrlexA1-87-fos zipper | 5 |
| pDP804 | ori p15A AmprlexA1-87408-jun zipper | 5 |
| plexFecA | pDP804 lexA1-87408-fecA1-79 | This study |
| plexAN | pMS604 lexA1-87 | This study |
| plexRC | pMS604 lexA1-87-fecR101-317 | This study |
| plex237 | pMS604 lexA1-87-fecR237-317 | This study |
| plex288 | pMS604 lexA1-87-fecR101-288 | This study |
| plex278 | pMS604 lexA1-87-fecR101-278 | This study |
| plexLZG | pMS604 lexA1-87-fecR227-288 | This study |
| pT7-7 | ori ColE1 phage T7 gene promoter | 28 |
| pAA70 | pT7-7 fecR | 34 |
| pLZ1A | pT7-7 fecR(L247A) | This study |
| pLZ1C | pT7-7 fecR(L247C) | This study |
| pLZ1 | pT7-7 fecR(L247P) | This study |
| pLZ2 | pT7-7 fecR(L254P) | This study |
| pLZ3 | pT7-7 fecR(L261P) | This study |
| pLZ4 | pT7-7 fecR(V268P) | This study |
| plexLZ1A | pMS604 lexA1-87-fecR101-317(L247A) | This study |
| plexLZ1C | pMS604 lexA1-87-fecR101-317(L247C) | This study |
| plexLZ1 | pMS604 lexA1-87-fecR101-317(L247P) | This study |
| plexLZ2 | pMS604 lexA1-87-fecR101-317(L254P) | This study |
| plexLZ3 | pMS604 lexA1-87-fecR101-317(L261P) | This study |
| plexLZ4 | pMS604 lexA1-87-fecR101-317(V268P) | This study |
| pFPV25 | ori ColE1 Ampr contains promoterless gfpmut3 | 30 |
| pGFPA′ | pFPV25 PfecA-gfp | This study |
| pGFPAA′ | pFPV25 fecA PfecA-gfp | This study |
| pHSG576 | pSC101 derivative, Cmr | 29 |
| pMMO203 | pHSG576 fecI fecR | 27 |
| pLCLZ1A | pHSG576 fecI fecR(L247A) | This study |
| pLCLZ1C | pHSG576 fecI fecR(L247C) | This study |
| pLCLZ1 | pHSG576 fecI fecR(L247P) | This study |
| pLCLZ2 | pHSG576 fecI fecR(L254P) | This study |
| pLCLZ3 | pHSG576 fecI fecR(L261P) | This study |
| pLCLZ4 | pHSG576 fecI fecR(V268P) | This study |
| pIS712 | pHSG576 fecI fecR fecA | 15 |
| pOR603 | pHSG576 fecI fecR(T2511C)(L254E) fecA | This study |
| pOR601 | pHSG576 fecI fecR(C2255G/T2256C)(L269G) fecA | This study |
| pOR600 | pHSG576 fecI fecR(T2600C/C2602G)(F284L) fecA | This study |
| pOR603D1 | pHSG576 fecI fecR(T2511C)(L254E) fecA(C3054A)(G39R) | This study |
| pOR601D1 | pHSG576 fecI fecR(C2255G/T2256C) (L269G) fecA(C3054A) (G39R) | This study |
| pOR600D1 | pHSG576 fecI fecR(T2600C/C2602G) (F284L) fecA(C3054A) (G39R) | This study |
| pOR603C3 | pHSG576 fecI fecR(T2511C)(L254E) fecA(G3004C)(D43E) | This study |
| pOR601C3 | pHSG576 fecI fecR(C2255G/T2256C) (L269G) fecA(G3004C) (D43E) | This study |
| pOR600C3 | pHSG576 fecI fecR(T2600C/C2602G) (F284L) fecA(G3004C) (D43E) | This study |
| pORD1 | pHSG576 fecI fecR fecA(C3054A) (G39R) | This study |
| pORC3 | pHSG576 fecI fecR fecA(G3004C) (D43E) | This study |
| pLCIRA | pHSG576 fecI fecR fecA | 27 |
| pIS135 | pHSG576 fecI fecR | 22 |
| pHCIR | pBCKS+fecI fecR1-110 | This study |
| pAS103 | pBCKS+fecI fecR | 27 |
| pMS604fecA1-79 | pMS604 lexA1-87-fecA1-79 | This study |
| pUS11 | pDP804 lexA1-87408-fecR101-317 | 7 |
| pHBlexR2 | pDP804 lexA1-87408-fecR101-317 (T2164A/T2340C) (D138E/V197A) | This study |
| pHBlexR7 | pDP804 lexA1-87408-fecR101-317 (A2235G/A2471G/A2619T) (D162G/S241G/D290V) | This study |
| pHBlexR12 | pDP804 lexA1-87408-fecR101-317 (A2115G/T2142C/A2145G/A2232G/A2322G/A2504G/T2628A) (Q122R/L131P/N132S/K161R/N191S/K252E/L293Q) | This study |
| pHBlexR13 | pDP804 lexA1-87408-fecR101-317 (T2445C/G2548A) (V232A/G267S) | This study |
| pHBlexR15 | pDP804 lexA1-87408-fecR101-317 (A2423G/A2615G) (S225G/T289A) | This study |
| pHBlexR16 | pDP804 lexA1-87408-fecR101-317 (C2616A/T2649C) (T289N/L300P) | This study |
| pHBlexR21 | pDP804 lexA1-87408-fecR101-317 (A2212T/T2421C/A2524G/T2655C) (E154D/F224S/I258M/V302A) | This study |
| pHBlexR22 | pDP804 lexA1-87408-fecR101-317 (G2067T/A2244G) (G106V/Q165R) | This study |
| pHBlcR2 | pHSG576 fecI fecR (T2164A/T2340C) (D138E/V197A) fecA | This study |
| pHBlcR7 | pHSG576 fecI fecR (A2235G/A2471G/A2619T) (D162G/S241G/D290V) fecA | This study |
| pHBlcR12 | pHSG576 fecI fecR (A2115G/T2142C/A2145G/A2232G/A2322G/A2504G/T2628A) (Q122R/L131P/N132S/K161R/N191S/K252E/L293Q) fecA | This study |
| pHBlcR13 | pHSG576 fecI fecR (T2445C/G2548A) (V232A/G267S) fecA | This study |
| pHBlcR15 | pHSG576 fecI fecR (A2423G/A2615G) (S225G/T289A) fecA | This study |
| pHBlcR16 | pHSG576 fecI fecR (C2616A/T2649C) (T289N/L300P) fecA | This study |
| pHBlcR21 | pHSG576 fecI fecR (A2212T/T2421C/A2524G/T2655C) (E154D/F224S/I258M/V302A) fecA | This study |
| pHBlcR22 | pHSG576 fecI fecR (G2067T/A2244G) (G106V/Q165R) fecA | This study |
| pHBhcR2 | pBCKS+fecI fecR (T2164A/T2340C) (D138E/V197A) | This study |
| pHBhcR7 | pBCKS+fecI fecR (A2235G/A2471G/A2619T) (D162G/S241G/D290V) | This study |
| pHBhcR12 | pBCKS+fecI fecR (A2115G/T2142C/A2145G/A2232G/A2322G/A2504G/T2628A) (Q122R/L131P/N132S/K161R/N191S/K252E/L293Q) | This study |
| pHBhcR13 | pBCKS+fecI fecR (T2445C/G2548A) (V232A/G267S) | This study |
| pHBhcR15 | pBCKS+fecI fecR (A2423G/A2615G) (S225G/T289A) | This study |
| pHBhcR16 | pBCKS+fecI fecR (C2616A/T2649C) (T289N/L300P) | This study |
| pHBhcR21 | pBCKS+fecI fecR (A2212T/T2421C/A2524G/T2655C) (E154D/F224S/I258M/V302A) | This study |
| pHBhcR22 | pBCKS+fecI fecR (G2067T/A2244G) (G106V/Q165R) | This study |
a
Subscripts denote amino acid residues of the encoded proteins. Numbers in parentheses indicate amino acids replaced.