TABLE 4.
Binding of mutated FecR101-317 to FecA1-79
| Plasmid | FecR protein | β-Galactosidase activitya (Miller units) |
|---|---|---|
| pDP804 | LexA1-87408-Jun | 636 |
| pUS11 | LexA1-87408-FecR101-317 | 134 |
| pHBLexR2 | LexA1-87408-FecR101-317 (D138E, V197A) | 93 |
| pHBLexR7 | LexA1-87408-FecR101-317 (D162G, S241G, D290V) | 542 |
| pHBLexR12 | LexA1-87408-FecR101-317 (Q122R, L131P, N132S, K161R, N191S, K252E, L293Q) | 607 |
| pHBLexR13 | LexA1-87408-FecR101-317 (V232A, G267S) | 516 |
| pHBLexR15 | LexA1-87408-FecR101-317 (S225G, T289A) | 537 |
| pHBLexR16 | LexA1-87408-FecR101-317 (T289N, L300P) | 688 |
| pHBLexR21 | LexA1-87408-FecR101-317 (E154D, F224S, I258M, V302A) | 754 |
| pHBLexR22 | LexA1-87408-FecR101-317 (G106V, Q165R) | 731 |
a
Determined by using the bacterial two-hybrid LexA-based system in E. coli SU202 sulA-lacZ, which, in addition to FecR derivatives, synthesized LexA1-87-FecR1-79 encoded by pMS604fecA1-79.