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Induction activity of FecR leucine motif mutantsa
| Plasmid | FecR protein | Relative fluorescence
|
|
|---|---|---|---|
| NB medium | NB medium + citrate | ||
| pHSG576 | None | 3 | 4 |
| pMMO203 | FecR | 22 | 356 |
| pLCLZ1A | FecR(L247A) | 21 | 285 |
| pLCLZ1C | FecR(L247C) | 21 | 235 |
| pLCLZ1 | FecR(L247P) | 18 | 107 |
| pLCLZ2 | FecR(L254P) | 25 | 28 |
| pLCLZ3 | FecR(L261P) | 10 | 36 |
| pLCLZ4 | FecR(V268P) | 11 | 174 |
Induction was determined in E. coli AA93 Δfec(pGFPAA′ fecA, PfecA::gfp). The plasmids listed encoded FecI in addition to the listed FecR derivatives.
