TABLE 6.
Induction activity of FecR mutantsa
| Plasmid | Protein | Relative fluorescence (% of wild-type)
|
Stability | |
|---|---|---|---|---|
| NB medium | NB medium + citrate | |||
| pIS135 | FecR | 7 | 8 | Wild type |
| pLCIRA | FecR | 17 | 100 | Wild type |
| pHBlcR2 | FecR(D138E, V197A) | 43 | 74 | As wild type |
| pHBlcR7 | FecR(D162G, S241G, D290V) | 7 | 8 | Unstable |
| pHBlcR12 | FecR(Q122R, L131P, N132S, K161R, N191S, K252E, L293Q) | 7 | 10 | As wild type |
| pHBlcR13 | FecR(V232A, G267S) | 22 | 18 | Unstable |
| pHBlcR15 | FecR(S225G, T289A) | 15 | 58 | As wild type |
| pHBlcR16 | FecR(T289N, L300P) | 16 | 14 | As wild type |
| pHBlcR21 | FecR(E154D, F224S, I258M, V302A) | 10 | 12 | Unstable |
| pHBlcR22 | FecR(G106V, Q165R) | 7 | 10 | As wild type |
a
Induction was determined in E. coli AA93 Δfec(pGFPA′ fecA, PfecA::gfp). The plasmids listed encoded FecA and FecI in addition to the listed FecR derivatives except pIS135, which encoded FecI but no FecA.