TABLE 7.
Induction activity of FecR and FecA mutantsa
| Plasmid | Protein | Relative fluorescence
|
|
|---|---|---|---|
| NB medium | NB medium + citrate | ||
| pHSG576 | None | 40 | 45 |
| pIS712 | FecR FecA | 65 | 856 |
| pOR603 | FecR(L254E) FecA | 67 | 70 |
| pOR601 | FecR(L269G) FecA | 59 | 66 |
| pOR600 | FecR(F284L) FecA | 58 | 63 |
| pOR603D1 | FecR(L254E) FecA(G39R) | 65 | 78 |
| pOR601D1 | FecR(L269G) FecA(G39R) | 63 | 318 |
| pOR600D1 | FecR(F284L) FecA(G39R) | 48 | 108 |
| pOR603C3 | FecR(L254E) FecA(D43E) | 65 | 139 |
| pOR601C3 | FecR(L269G) FecA(D43E) | 65 | 124 |
| pOR600C3 | FecR(F284L) FecA(D43E) | 47 | 289 |
| pORD1 | FecR FecA(G39R) | 45 | 801 |
| pORC3 | FecR FecA(D43E) | 43 | 764 |
a
Induction was determined in E. coli AA93 Δfec(pGFPA′ fecA, PfecA::gfp). The plasmids listed encoded FecI in addition to the listed FecR and FecA derivatives.