TABLE 2.

Muropeptide identities and quantification from peptidoglycan of B. subtilis HR (wild type), GJH161 (lytH), and GJH171 (lytH cwlD)

Muropeptidea	Identity	Concn (mol%) in:
		HR (wild type)	GJH161 (lytH)	GJH171 (lytH cwlD)
1	Disaccharide tripeptide	4.6	4.4	1.9
2	Disaccharide alanine	6.2
3	Disaccharide tetrapeptide	13.2	21.2	91.4
4	Tetrasaccharide alanine with open lactam	1.2
5	Tetrasaccharide tetrapeptide with open lactam	0.9	5.3
6	Tetrasaccharide alanine with reduced lactam	3.1
7	Tetrasaccharide tetrapeptide with reduced lactam	4.5	10.7
8	Disaccharide tripeptide disaccharide tetrapeptide	0.9	1.0	0.6
9	Disaccharide tetrapeptide disaccharide tetrapeptide	0.6	1.0	5.3
10	Tetrasaccharide tetrapeptide	21.5	36.0
11	Tetrasaccharide alanine	21.7
12	Hexasaccharide tetrapeptide with 1 reduced lactam	1.3	2.3
13	Disaccharide tetrapeptide tetrasaccharide tetrapeptide	1.6	1.9
14	Hexasaccharide alanine with 1 reduced lactam	1.3
15	Hexasaccharide alanine with 1 reduced lactam	1.3
17	Hexasaccharide tetrapeptide with 1 reduced lactam	1.4	2.1
19	Tetrasaccharide tetrapeptide tetrasaccharide tetrapeptide	0.8	1.0
20	Hexasaccharide tetrapeptide	6.7	11.5
21	Hexasaccharide alanine	6.0
22	Tetrasaccharide tetrapeptide hexasaccharide tetrapeptide	0.3	0.5
23	Octasaccharide tetrapeptide	0.5	1.1
24	Octasaccharide alanine	0.4
A	Disaccharide tripeptide disaccharide tetrapeptide with 1 amidation			0.8

^aMuropeptides are numbered as indicated in Fig. 5.