TABLE 2.
Effect of blocking cell division on binding of cephalexin to PBP3 in growing cellsa
| Strain | Cell division target or mutation | % of PBP3 acylated by cephalexinb in:
|
No. of independent expts | |
|---|---|---|---|---|
| Dividing cells | Fila- ments | |||
| EC906 | FtsZ (induces sulA) | 62 | 25 | 1 |
| EC297 | ftsA12(Ts) | 76 ± 3 | 43 ± 8 | 7 |
| EC433 | ftsQ1(Ts) | 77 ± 3 | 52 ± 4 | 5 |
| JOE170 | FtsQ depletion | 78 | 52 | 1 |
| EC912 | FtsW depletion | 87 | 57 | 1 |
| JOE565 | FtsN depletion | 71 ± 12 | 34 ± 6 | 3 |
| Unweighted avg | 75 | 44 | ||
a
Cephalexin was used at a concentration of 10 μg/ml, and Bocillin FL counterlabeling was done with spheroplasts rather than envelopes prepared by detergent extraction as in Fig. 2.
b
Mean ± standard deviation given for strains EC297, EC433, and JOE565 for which more than one experiment was performed.