FIG. 1.

(A) The identical genetic organization of the nbz genes encoding NBDO from strain JS765 and ntd genes encoding 2NTDO from strain JS42. Arrows indicate the direction of transcription. The locations of primer sets and the amplified DNA fragments for RT-PCR are designated RT1 and RT2. (B) RT-PCR amplification of the nbz (lanes 2 to 10) and ntd (lanes b to j) gene clusters. Lanes 2, 5, and 8, RT-PCR products from total RNA from succinate-grown JS765; lanes 3, 6, and 9, RT-PCR products from total RNA from JS765 grown with succinate plus nitrobenzene; lanes 4, 7, and 10, RT-PCR products from total RNA from JS765 grown on succinate plus salicylate; lanes b, e, and h, RT-PCR products from total RNA from succinate-grown JS42; lanes c, f, and i, RT-PCR products from total RNA from JS42 grown with succinate plus 2NT; lanes d, g, and j, RT-PCR products from total RNA from JS42 grown with succinate plus salicylate. Samples in lanes 5 to 7 and e to g were without RT. Lanes 2 to 7 and b to g were amplifications performed with the RT1 primer set, and lanes 8 to 10 and h to j were with the RT2 primer set. Lanes 1 and a were loaded with a 1-kb ladder (Invitrogen, Carlsbad, Calif.).